CORRELATION OF BLOOD MIRNA-221 EXPRESSION WITH DIFFERENT PATHOLOGIC LEVELS OF HELICOBACTER PYLORI INFECTION

Background and Aim:Helicobacter pylori (H. pylori), plays a major role in the development of gastric cancer. miRNAs including miRNA-221 are small single stranded non-coding RNA, that their expression level may change in different conditions including H. pylori infection.So, the aim of this study was evaluating the expression variation of miRNA- 221 among endoscopic candidates with or without H. pylori infection.Methods:50 biopsy samples and 50 blood samples were collected from endoscopic candidates who referred to Taleghani hospital of Tehran during 2016-17. mi-RNA extraction and cDNA synthesis was done based on the manufacturer protocol of Bio Basic and Intron products, respectively. The variation of mi-RNA 221 expression was evaluated by Quantitative Real-time PCR. Statistic analysis was done with SPSS version 17.Results:The gender of candidates included of 15 males and 35 females and the average age was 18 to 85 Based on the pathologic examination, patients were divided to H. pylori negative and positive infected groups. Also, by Real Time PCR, expression change of miRNA-221 level in H. pylori infected was evaluated and based on the results, the level of miRNA-221 expression changes 2 times more in H. pylori infected Vs. to negative candidates.Conclusion:Based on the results, it seems that blood miRNA -221 can also serve as a biomarker for the early diagnosis.

کلیدواژه ها:

miRNA ، Helicobacter pylori ، biopsy ، gastric cancer