THE EXPRESSION OF RECOMBINANT HYDI PROTEIN OF ECHINOCOCCUS GRANULOSUS IN E. COLI BL21 (DE3) STRAIN

Background and Aim:In Intermediate hosts such as humans and livestock where the eggs of Echinococcus granulosus develop into the metacestode (larval) stage, cause cystic echinococcosis (CE). Hydatid cyst is usually located in the liver and lungs, however rare cases showing localization of the cyst in other organs or tissues. Our objective was to investigate further the role of E. granulosus antigen B (AgB) in human early inflammatory response. An accurate diagnosis and early treatment of CE is crucial and can reduce the severity of infection. HydI is one of the antigens that can be used for detection of CE antibody in infected patients.Methods:HydI gene was sub-cloned in expression plasmid pET31b. The recombinant plasmid was then transferred to E. coli Bl21 (DE3) strain. Then the recombinant protein expression was analyzed by SDS-PAGE.Results:The presence of HydI gene fragment in the recombinant plasmid was confirmed by NdeI/XhoI enzymes. Sequence analysis of the correct cloning, revealed, %100 homology with the published sequence of HydI gene. SDS-PAGE analysis showed expression of protein band at 9.33 KDa in induced bacteria.Conclusion:In conclusion, this study reported construction of a plasmid DNA encoding HydI protein of E. granulosus. We confirmed that the plasmid pET31b and the BL21 (DE3) expression host are able to direct synthesis of antigenic HydI protein.

کلیدواژه ها:

Echinococcosis ، HydI ، expression ، Bl21 (DE3)