STUDY OF THE ABILITY OF BIOFILM FORMATION IN ESCHERICHIA COLI ISOLATES FROM URINARY TRACT INFECTIONS (UPEC) BY MICROTITER PLATE

Background and Aim:Urinary tract infections are one of the most common infections and Escherichia coli is one of the main causes of this infection. A Biofilm is actually a microbial collection that its structure makes the bacteria more resistant and reduces the treatment of these infections. The aim of this study is determine the ability of biofilm formations in E. coli isolated from urinary tract infections.Methods:In this study, 72 samples of Escherichia coli isolated from urinary tract infections were collected from hospitals in Mashhad in 1393-1396 and were approved by Biochemical tests and then ability to biofilm formation was studied by microtiter plate and was read using an ELISA reader.Results:Based on optical density from the 72 studied isolates, 5 isolates (7%) had the ability to form a strong biofilm, 18 (25%) isolates had the ability to form moderate biofilms, 37 (51%) isolates, had the ability to form weak biofilms and 12 (17%) isolates did not have the ability to form biofilm.Conclusion:Considering the high percentage of biofilms formation (83%) in the isolates, it is recommended that Suitable hygiene items to be used.