EVALUATION OF NLRP1 GENE EXPRESSION IN PATIENT WITH SEPTICEMIA AND CONTROLS

Background and Aim:NLRP1 is intracellular receptor that recognition the microorganism dependent molecular patterns. Septicemia is a condition in which the infection has entered the patient s blood stream. The main intracellular mechanism of anti-septicemia is still being investigated. Therefore, in this study, NLRP1 gene expression was evaluated in septicemia subjects in comparison with control subjects.Methods:The expression of NLRP1 gene in 40 patients with septicemia was mediated by Real time PCR was evaluated. The bacterial agents present in the blood of septal patients were determined by blood culture.Results:Results showed that expression of NLRP1 gene in the mRNA level in patients was significantly increased compared to healthy controls. Blood culture results were infected by reporting four bacteria of Escherichia coli, Staphylococcus aureus, Acintobacter Bumanni and Pseudomonas aeruginosa. The expression of NLRP1 genes in patients with different bacterial infections did not differ.Conclusion:Based on the results, it seems it appears that NLRP1 is the main intracellular immunity against bacteria during sepsis they can be investigated for their role in stimulating the immune system through the activation of inflammatory cytokines, Interleukin 1 and 18. It is hoped to help medico with new therapies for sepsis, including immunotherapy, cell therapy and gene therapies.

کلیدواژه ها:

Septicemia ، NLRP1 ، Gene expression