PRODUCTION OF ANTIBIOTIC GRISEOFULVIN (GSF) FROM NATIVE NIGROSPORA SP

Background and Aim:Production of antibiotic GSF from endophytic fungi, Nigrospora has been investigated in this project. GSF is a non-toxic antibiotic showing activity against pathogenic bacteria and fungi. This is a first report of griseofulvin producing native and endophytic Nigrospora sp. which was isolated from Iran.Methods:The strains were supplied from Iranian Biological Resource Center (IBRC) by the following deposition numbers: IBRC30242, IBRC30268, IBRC30269 and IBRC30199 and identified based on the morphological characterizations and ITS-rDNA sequence comparison. To produce GSF, fungi mycelia were inoculated in to 500 ml Erlenmeyer flasks containing 100 ml potato dextrose broth and incubated at 25 ºC on a rotary shaker (200 rpm) for 21 days. For harvesting mycelia, the fermentation broth was centrifuged at 5000g for 15 min. For extraction of GSF, The biomass was subjected to 100 ml ethyl acetate for 3h on rotary shaker (220 rpm).Results:The liquid phase was recovered by filter paper Whatman No. 41 and then concentrated under low oxygen pressure. Also the presence of GSF in the extract was confirmed by spectrophotometry at 290 nm. Activity of extract was evaluated against S. aureus, E. coli and phytopathogenic fungus Fusarium sp. by agar disc diffusion method.Conclusion:GSF is one of the first antifungal natural products which used for treatment of mycotic disease such ringworm infections. This antifungal antibiotic has a wide variety of biological activities. It’s anticipated that endophytic fungi comes to be a superb sources for bioactive compound.

کلیدواژه ها:

Endophytic ، Nigrospora ، Secondary metabolite ، Antifungal antibiotics ، Griseofulvin