QUALITATIVE SCREENING AND LIPID EXAMINATION OF BACTERIAL CELLS BY COLORING USING A NILE RED

Background and Aim:Coloring with Nile red as a method for determining the qualitative amount of lipid in many studies has been used on lipid accumulation in microorganism cells. But application of this technique has not been reported so far to investigate the production of lipid in fat producing cyanobacteria strain cell to produce biodiesel. This study is aimed to provide a quick and practical method for fat producing cyanobacteria strain screening process.Methods:To prepare a Nile red solution for coloring, solution of 33% gr / lit was prepared in acetone solution of this material. In order to color using Nile red 1 ml of cell suspension was centrifuged at 5000 rpm and washed twice with 1 ml of phosphate buffer solution, again 1 ml of buffer solution was added to it. After homogenization, 10 micro-liters of Nile red solution was added to it. Obtained suspension was placed in dark for 5 minutes at 37 ° C and then it was observed using a fluorescent microscope.Results:Coloring with Nile red to investigate the production of intracellular cyanobacteria cell oil, was conducted to produce biodiesel. The location of intracellular lipid accumulation was determined by golden fluorescence color, which was caused by emission of light from fluorescent color of Nile red after binding to oily compounds produced in fat-producing cyanobacteria cells.Conclusion:: Screening fat producing cyanobacteria strains using a coloring method with Nile red fluorescent has a very good efficiency.

کلیدواژه ها:

Biodiesel ، Cyanobacteria ، Nile red coloring