ISOLATION AND IDENTIFICATION OF IRON RESISTANT BACTERIA FROM PARDIS MOUNTAIN IN JAM AREA AND FAJR JAM GAS REFINERY COMPANY WASTEWATER AND STUDY OF HEAVY METAL BIOREMEDIATION BY THEM

Background and Aim:Fe (iron) is an essential element for human and other organism’s cell activity but in high concentration have toxic effects on biosystemes. Heavy metals even at low concentration in environment, soil and water, due to their pollution effects could been harmful for humans, animals and plants.Methods:Heavy metals tolerant micro-organisms can bioremediate heavy metals from their environment. in this study 21 iron tolerants strains of micro-organisms were isolated from water of subterranean canal and soil of Pardis mountain in Jam area and Fajr Jam Gas Refinary Company (F.J.G.C) wastewater.Results:they were tolerant to high concentration of iron (up to 140 gr /lit) and they could remediate high perecent of iron. 4 strains of these micro-organisms have selected for identification. 16 Sr DNA identification results of these strains showed that 2 striains (LG3B1 and LG52B) that isolated from wastewater of (F.J.G.C) were the same and identified as (Bacillus paramycoides. NH24A2(T) ) and (KP2A1 and KP4B2) isolated from Pardis mountain were as (Pseudomonas stutzeri ATCC 17588(T) and Pseudomonas songnenensis NEAU-ST5-5(T) respectively.Conclusion:Iron and other metal enter human and all living cells through the uptake pathways by siderophore, so iron resistance micro-organisms could be used for pollutants bioremediation. It could be used the consercium of these 21 strain for heavy metal bioremediation.

کلیدواژه ها:

bioremediation ، Heavy metals ، iron ، Pseudomonas stutzeri ، Pseudomonas songnenensis ، Bacillus paramycoides