MOLECULAR ISOLATION OF RHIZOPUS ORYZAE STRAINS WHICH PRODUCE LIPASE FROM NATURAL SOURCES

Background and Aim:Introduction and objectives: Aspergilla genus includes fungi which are applied industrially. Some of the strains of this genus lead to the infection of agricultural crops like pistachio, corn and other crops and besides to the reduction of cultural crops quality, they have led to the poisoning and mutagenesis of other creatures like human. The goal of this study is molecular isolation of Rhizopus oryzae strains which are producing lipase from natural sources.Methods:The prepared samples were cultured on PDA medium. To produce these enzymes, mineral medium was used for solid-state fermentation. In following, the effects of carbon source, the initial pH of the medium and incubation temperature on the production of enzyme were assayed. After production of spore suspension, solid state fermentation was carried out. The enzymes were isolated after the fermentation and their enzymatic activities were evaluated. ...Results:The Rhizopus oryzae genus showed high enzymatic activity for lipase in most of its strains and are able to produce lipase enzyme and regarding the vast expansion of this fungus in this country, it can be an important genus for biotechnology and industry driven activities. Based on the present screening, most of the local strains are able to produce the lipases ...Conclusion:The fungi have a significant biotechnological potential in the production of enzymes and some of them are able to produce the enzymes in large industrial scale. The results about the lipase activity of ...

کلیدواژه ها:

Enzyme ، DNA extraction ، Lipase ، Aspergillus ، Isolation