RELATION OF TOXA AND TOXS GENES AND ANTIMICROBIAL RESISTANCE IN PSEUDOMONAS AERUGINOSA

Background and Aim: Pseudomonas aeruginosa is one of the most important nosocomial pathogens that causes opportunistic infections in human. It can tolerate a wide variety of antibiotics by different resistance mechanisms Such as efflux pupms. P. aeruginosa also possesses several virulence factors such as exotoxin A (toxA) and exoenzyme S (toxS). The study aim to show the relation between presence of toxA and toxS genes and antimicrobial resistance in this bacteria.Methods:At first, species identify using biochemical tests, and their antibiogram pattern obtain according to CLSI standards. Antimicrobial susceptibility testing will be done by disk diffusion method and Then The presence of toxA and tox s genes will investigate using PCR (Real-time PCR or multiplex pcr)Results:results show that there is a relationship between antimicrobial resistance and presence of virulence factors, such as exotoxin and exoanzime, most of resistant isolates have these exotoxin genes . this relationship can be an important warning to treatment centers in terms of control of disease caused by pseudomonas aeroginosa.Conclusion:P. aeruginosa as one of the most common cause of nosocomial infections exhibits remarkable ability to acquire resistance to antimicrobial agents and MDR P. aeruginosa nosocomial infections are increasingly recognized worldwide .toxA and toxS both genes are almost contribute to pathogenicity and antimicrobial resistance in isolates of P. aeruginosa and studies show that there is relationship between presence of these genes and antimicrobial ability.

کلیدواژه ها:

Pseudomonas aeruginosa ، toxA and toxS ، Antimicrobial Resistance