ANTIBACTERIAL EFFECTS OF COPPER DIOXIDE AND TITANIUM DIOXIDE NANOPARTICLES ON ENTEROBACTER AEROGENES BACTERIA IN SOLID MEDIUMS

Background and Aim:High incidence of infectious diseases and the growing resistance of bacteria against traditional antibiotics has led to widespread investigation of alternative antibacterial compounds such as nanoparticles. The current study was conducted in vitro to investigate impact on growth of CuO and TiO2 nanoparticles on Enterobacter aerogenes bacteria in solid mediums.Methods:In solid media, well diffusion and disk diffusion methods (In five concentrations 10, 50, 100, 250 and 500 ppm) and cylinder diffusion method were used 50, 250, 500, 1250, 2500 ppm concentrations of nanoparticles. In this test, for better measurement of zone of inhibition, Triphenyl tetrazolium chloride (TTC) were used. Plates incubated at 37 °C for 24 h. Inhibition zones measured by caliper subtly and reported.Results:In well diffusion, disk diffusion and cylinder diffusion tests, the diameter of inhibition zone of Copper oxide nanoparticles was larger than the Titanium oxide inhibition zone. Copper oxide nanoparticles could form a non-growth zone at all concentrations other than the initial concentration. But titanium dioxide nanoparticles could not form any inhibition zone.Conclusion:Bactericidal property of CuO nanoparticles was remarkably higher than TiO2 nanoparticles in the same condition. The concentration of nanoparticles has a direct impact on bactericidal ability. Possibly by more research and investigation, CuO nanoparticles can be consider as adjunctive therapy in combination with other antibacterial compounds.

کلیدواژه ها:

Enterobacter aerogenes ، Copper oxide ، Titanium dioxide ، Nanoparticles ، Antibacterial compounds