COPPER OXIDE AND TITANIUM DIOXIDE NANOPARTICLES IMPACT ON GROWTH OF ENTEROBACTER AEROGENES IN LIQUID MEDIUMS

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 408

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شناسه ملی سند علمی:

MEDISM19_230

تاریخ نمایه سازی: 13 مهر 1397

چکیده مقاله:

Background and Aim:High incidence of infectious diseases and the growing resistance of bacteria to traditional antibiotics has led to widespread investigation of alternative antibacterial compounds such as nanoparticles. The current study was conducted in vitro to investigate Impaction of CuO and TiO2 nanoparticles on Enterobacter aerogenes growth in liquid medium.Methods:Enterobacter aerogenes bacterium ATCC:13048 was purchased from microbiological reservoir of the Pasteur Institute of Iran. CuO nanoparticles with a size of 40 nm and 10 to 25 nm TiO2 provided from US Research Nanomaterials. To do this test following incubation of bacteria and different concentrations of nanoparticles, optical density of cultures were monitored for five hours-every half hour. At 30, 60 and 120 minutes after incubation, colony forming unites of culture was determined.Results:In the liquid test; Copper oxide nanoparticles significantly decreased the CFU of Enterobacter aerogenes in comparison to Titanium dioxide nanoparticles, while the measurement of optical density (OD) of bacteria and nanoparticle incubations were slightly different. We assumed that, TiO2 optical properties interfere with OD monitoring assay and did not allow reading real values during time of incubation.Conclusion:Bactericidal property of CuO nanoparticles was remarkably higher than TiO2 nanoparticles in the same condition. The concentration of nanoparticles has a direct impact on bactericidal ability. Possibly by more research and investigation, CuO nanoparticles can be consider as adjunctive therapy in combination with other antibacterial compounds.

نویسندگان

Shervin Shabani

Department of Microbiology, Urmia branch, Islamic Azad University, Urmia, Iran

Hamed Charkhian

Department of Biotechnology, Urmia branch, Islamic Azad University, Urmia, Iran

Ehsan Soleimannezhadbari

Young Researchers Club, Urmia Branch, Islamic Azad University, Urmia, Iran

Ali Ghahremany

Microbiology Lab ,Urmia Branch, General Bureau of Standards West Azerbayjan, Urmia, Iran