IDENTIFICATION AND ANTIBIOGRAM ANALYSIS OF VARIOUS BACTERIAL ISOLATES FROM URINE IN ROUTINE DIAGNOSTIC LOBORATORIES OF KERMAN, IRAN

Background and Aim:Drug resistance is a burning issue for bacterial isolates and inappropriate use of antibiotics is one of the important factors of increasing of resistance. The aim of this study were determination and comparison of antibiotic susceptibility pattern among isolates from urine.Methods:Totally 104 samples were collected from urinary tract infections. The bacterial isolates were identified by gram’s staining. Conventional biochemical tests were used for detection of bacteria. Antimicrobial susceptibility tests were performed using the Kirby-bauer disc diffusion method.Results:The results revealed that escherichia coli (48%) was the most common bacterial isolate followed by staphylococcus spp. (22%), klebsiella spp. (18%) and proteus spp. (12%). Antibiogram results shown that the most resistant antibiotic to e.coli was (ampicillin 78%), staphylococcus spp. (penicillin 83%), klebsiella spp. (amoxicillin 100%) and proteus spp. (sulphamethoxazole 67%). Also the most effective antibiotic to e.coli was (fosfomycin 96%), staphylococcus spp. (fosfomycin 65%), klebsiella spp. (gentamicin 84%) and proteus spp. (gentamicin 67%).Conclusion:Level of resistance in referred isolates were very high. It is very important to reduce frequent misuse, inadequate dosage and easy availability of antimicrobials to avoid antibiotic resistance.

کلیدواژه ها:

antibiogram ، antibiotic resistance ، urinary infections ، biochemical methods