THE PREVALENCE OF PANTON VALENTINE LEUKOCIDIN POSITIVE IN S. AUREUS ISOLATED FROM SKIN AND SOFT TISSUE INFECTION

Background and Aim:Panton Valentine Leukocidin (PVL) has been linked with invasive Staphylococcus aureus skin and soft tissue infections. Aim: To determine the prevalence of Panton-Valentine leukocidin gene and antibiotic susceptibility pattern of S. aureus isolated from skin and soft tissue infection (SSTI).Methods:A cross-sectional study was conducted in 400 patients with skin and soft tissue infection in Razi skin Hospital, Tehran, Iran. Sterilized swab was used to collect samples of skin infections. Staphylococcus aureus isolates were identified using standard biochemical tests. The susceptibility of isolates to different antibiotics was determined by disk diffusion method according the guidelines of the Clinical and Laboratory Standards Institute (CLSI). PCR was used to investigate the presence of mecA and pvl gene.Results:A total Of the 400 isolates were collected from patients referred to Razi skin Hospital in Tehran, Iran. 205 out of 400 isolates (51.3%) were S. aureus, 96(46.8%) isolates were methicillin-resistant staphylococcus aureus (MRSA). 136 out of 205 patients with S. aureus infection (66.3 %) had bullous pemphigoid and pemphigus skin disease. 94.6% of the isolates were penicillin resistant, all of the isolates showed sensitivity to vancomycin, linezolid and 98% of the isolates were susceptible to daptomycin. 116(56.6%) of isolates were MDR (Multi Drug Resistance).Conclusion:in this study, more than half of the skin and soft tissue infections caused by MDR S.aureus. The presence of pvl in MRSA isolates was high.

کلیدواژه ها:

S.aureus ، MRSA ، SSTI ، pvl