BACTERIAL BIOFILM IN VENTILATOR-ASSOCIATED PNEUMONIA

Background and Aim: Introduction Development of Ventilator-associated pneumoniae (VAP) presents an intricate chemistry comprising of intubation and invading bacteria which often are tied up with biofilm production Aims To assess the phenotypic and genetic basis of biofilm formation by isolated from Acinetobacter baumannii and klebsiella pneumoniae mechanically ventilated and VAP developed patients.Methods:Materials and Methods Endotracheal aspirate obtained from 40 mechanically ventilated patients were collected for culture at least in four episodes including, 48 hours, 72 hours, 7 days and 10 days after being ventilated. . Isolates were identified using standard phenotypic methods and the antibiotic susceptibility of them was determined by agar diffusion method. The ability of isolates to form biofilms was evaluated quantitatively using micro titer plate method and finally, the presence of biofilm genes was investigated by molecular method.Results:The prevalence of VAP was 15%. A.baumannii (n=68) K. pneumoniae (n=32). 60.2% A.baumannii produced either moderate or strong biofilm, whereas 67.7% K.pneumoniae, isolates revealed either moderate or strong biofilm on micro titer plate. In the present investigation, 57.4% of A.baumannii showed the presence of bap gene producing product of 400bp. Gene type3 product of 817bp was present in all K. pneumoniae isolates while, mrkA product of 328bp and fimK product of 597bp genes were shown in 65.6% and 81.2% isolates respectively by multiplex PCR.Conclusion:Drug resistance phenotypes require specific national and regional therapeutic studies focusing on antimicrobial stewardship programs. Presence of biofilm requires routine surveillance of VAP, to track endemic VAPs.

کلیدواژه ها:

Ventilator-associated pneumonia- ، Biofilm