INVESTIGATION OF THE CHARACTERISTICS AND EFFICIENCY OF LYTIC PHAGE MDRACINETOBACTERBAUMANNII SEPARATED FROM THE ICU

Background and Aim:Introduction: Acinetobacterbaumannii is resistant to most commonly used antibiotics due to inherent mechanisms.Therefore, today it is considered as one of the most important bacteria in hospital infections, especially in ICU (intensive care unit). The increasing rate of multi-drug-resistant (MDR) bacteria has led to a renewed focus on alternative therapies, especially phage therapies.Bacteriophages are viruses that specifically use bacteria as their host. Aim: The purpose of this study is isolation, characterization and evaluation of the efficacy of Acinetobacterbaumannii (MDR)phage, so theyare good choice to substitutewith antibiotic treatment as an effective disinfectant or disinfecting the hospital.Methods:In a cross-sectional study in 1395, 84 samples of Acinetobacterbaumannii isolated from patients admitted to the intensive care unit. At first, the samples were identified by biochemical method and then amplified with blaOXA-51 gene. Then,antibiotic resistance patterndeterminedwith a diffuse method according to the CLSI instruction and finally separation of phage from water sources occurred.Results:the result of antibiotic resistance showed, 82% Amikacin, 97% Cefepime, 96% Ceftazidim, 99% Ciprofloxacin and 86% resistance to Rifampin.In the sewage water sample, the phage was isolated and Effective on Acinetobacterbaumanniiby creating plaque.Conclusion:Due to the resistance of Acinetobacterbaumannii to the majority of antibiotics,Phage Therapy could be the right candidate for Consideringsubstitute antibiotics to treat infections of this bacterium.

کلیدواژه ها:

Acinetobacterbaumannii ، Bacteriophage ، Phage Therapy ، Antibiotic Resistance