COMPARISON OF GREEN AND BLACK TEA EXTRACT ON THE DOMINANT PATHOGENS OF ENTERIC

Background and Aim:Green tea leaf (Camellia sinensis) contains polyphenolic compounds with antibacterial effects, thus studying its inhibitory effect on gastrointestinal pathogenic bacteria is very important. In this study, the inhibitory effects of aqueous extract of green and black tea leaves on Pseudomonas aeruginosa and Escherichia coli were compared.Methods:: In this study, the aqueous extract of the samples was taken by soxhlet, then diluted by 10% by means of a blank disc and disc diffusion agar method with equal dilutions of tea extract, Muller Hinton Agar culture medium and the same Parameters for 18 hours in the vicinity of the bacteria were incubated.Results:Black tea extract on E. coli bacteria with a growth avoidance hole with diameter of 25 mm and the same extract were determined on Pseudomonas aeruginosa with a hole diameter of 22 mm. Also, the effect of green tea extract on E. coli a diameter of 18 mm was applied to Pseudomonas aeruginosa with a diameter of 15 mm.Conclusion:Black tea extract has a more effective inhibitory effect on compare green tea extract, Which can reduce the risk of intestinal infections, and can also be used to condense this extract and use black tea polyphenols as an anti diarrhea syrup, In the pharmaceutical industry.

کلیدواژه ها:

Camellia sinensis ، Disc diffusion agar ، Inhibitory effect ، Pseudomonas aeruginosa ، E. coli