ANTIBACTERIAL ACTIVITY OF NIGELLA SATIVA EXTRACT AGAINST TWO PATHOGENIC BACTERIA

Background and Aim:Background: Medical herbs with anti microbial activity have always been important in traditional medicine and might be useful in antibacterial activity against the many pathogenic bacteria cause serious infections. The aim of this study was to determine the antibacterial activity of methanolic extract from Nigella sativa against 2 pathogenic bacteria in vitro.Methods:Methods: At first the seed of Nigella sativa extracts were prepared by the methanolic wetting extraction method in five different concentrations (400, 200, 100, 50, 25µg) and then its antibacterial activity against 2 standard strain bacteria (Escherichia coli or E.coli and Pseudomonas aeruginosa) was tested for the determination of MIC (minimum inhibitory concentration) and inhibitory zone diameter (ZOI) using disk diffusion method and agar serial dilution assays. Also the antibacterial activity of 11 antibiotics such as chloramphenicol, trimethoprim, streptomycin, gentamicin, erythromycin, doxycycline, nalidixic acid, ampicilline and est. was tested by the disk diffusion method.Results:Results: Statistical methods were using to analyze the data. The results demonstrated that the Nigella sativa methanolic extracts been effective against the 2 standard strain bacteria. (E.coli and Pseudomonas aeruginosa) .The methanolic extract of Nigella sativa was highly effective with 400 µg concentration. The methanolic Nigella sativa extract exhibited greater antibacterial activity than all of the selective antibiotics,Conclusion:Conclusion: This study demonstrates that methanolic extracts from the combination of the Nigella sativa constituents have excellent antibacterial activity against the 2 standard strain pathogenic bacteria. Further investigations will be necessary.

کلیدواژه ها:

pathogenic bacteria ، Antibacterial activity ، Nigella sativa