INVESTIGATION OF BETA-LACTAM ANTIBIOTIC RESISTANT E.COLI ISOLATED FROM URINARY TRACT INFECTIONS (UTI) PATIENTS REGARDING SHV, CTX-M AND IMP GENES

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 409

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شناسه ملی سند علمی:

MEDISM19_131

تاریخ نمایه سازی: 13 مهر 1397

چکیده مقاله:

Background and Aim:The resistance of E.coli to beta-lactam antibiotics is related to the acquisition of plasmids that encode broad-spectrum beta-lactamases. Extended-spectrum β-lactamases (ESBLs) have particular importance in antimicrobial therapyMethods:In this study the ferquency and role of SHV, CTX-M and IMP genes in resistance to Ceftriaxone, Imipenem and Piperacillin antibiotics in E.coli isolated from urinary tract infections (UTI) were determined. In total 270, non-duplicate E.coli producing ESBL were collected from the hospitilized patients of two genders in all ages with UTI at hospital of Damghan, Iran. The samples were isolated using screening test and double disc phenotypic test. All isolates were screened for the detection of SHV, CTX-M and IMP genes using PCR.Results:Among them 44.4% were positive for ESBLs which include 30% CTX-M gene, 27.5% SHV and 0% IMP gene. Results showed that the responses of male and Females patients to examind antibiotic was different. The distribution of underlying diseases also was different in UTI patientsConclusion:However, ESBLs producing strains of bacteria such as E.coli should be considered as a major threat for consumption of broad-spectrum cephalosporins. Therfore, overused antibiotic should be avoided to prevent resistance to them.

کلیدواژه ها:

antibiotic resistant ، E.coli ، urinary tract infections (UTI) ، CTX-M ، SHV

نویسندگان

Anna Abdolshahi

Food Safety Research Center (salt), Semnan University of Medical Sciences, Semnan, Iran

Aliakbar Shabani

Department & Center for Biotechnology Research, Semnan University of Medical Sciences, Semnan, Iran

Zahra Aminian

Damghan Velayat Hospital, Semnan University of Medical Sciences, Semnan, Iran

Tahereh Zinati

Student Research Committee, Semnan University of Medical Sciences, Semnan, Iran