IN VITRO ANTIMICROBIAL ACTIVITY OF CINNAMON, GARLIC, AND GINGER EXTRACTS ON METALLO-Β-LACTAMASE-PRODUCING PSEUDOMONAS AERUGINOSA: A POTENTIAL THERAPEUTIC APPROACH

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 458

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شناسه ملی سند علمی:

MEDISM19_129

تاریخ نمایه سازی: 13 مهر 1397

چکیده مقاله:

Background and Aim:Metallo-β-lactamase (MBL)-producing Pseudomonas aeruginosa is a leading cause of nosocomial infections, especially in burn patients worldwide. The antimicrobial properties of Cinnamomum verum, Allium sativum, and Zingiber officinale known as cinnamon, garlic, and ginger, respectively have not yet been reported in clinical isolates of P. aeruginosa producing metallo-β-lactamase. The present study was aimed to detect MBL genes and evaluate the inhibitory effect of cinnamon, garlic and ginger extracts on MBL-producing P. aeruginosa.Methods:Antibiotic resistance pattern of MBL-producing P. aeruginosa isolates was evaluated by Kirby-Bauer disk diffusion method. MBL-producing isolates were phenotypically tested by combined disk test (CDT). The prevalence of blaVIM-1 and blaIMP-1 genes encoding metallo-β-lactamase were detected by PCR. Minimum inhibitory concentration (MIC) of the acetonic, methanolic, and chloroformic extracts of cinnamon, garlic, and ginger on MBL-producing isolates was evaluated by the broth microdilution method.Results:81 out of 95 (85.2%) imipenem resistant P. aeruginosa isolates were MBL-producing strains. 13 out of 81 (16.04%) and 18 out of 81 (22.22%) MBL-producing P. aeruginosa were positive for blaIMP-1 and blaVIM-1 genes, respectively. The inhibitory concentrations of the acetonic, methanolic, and chloroformic extracts of cinnamon, garlic, and ginger ranged from ≥1.50 mg/ml to ≥12.50 mg/ml.Conclusion:We find that the methanolic extract of cinnamon and garlic as well as the acetonic extract of ginger has significant antibacterial activity against the MBL-producing P. aeruginosa. This medicinal plants can be considered as a source of forgotten antimicrobial agents to avoid treatment failure and mortality.

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نویسندگان

Neda Yousefi

Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Gita Eslami

Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Ali Hashemi

Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Mehrzad Sadredinamin

Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran