THE EFFECT OF THE DEHYDROZINGERONE ON THE BIOFILM FORMATION IN CANDIDA ALBICANS

Background and Aim:: Candida albicans is an important fungal pathogen of humans which is responsible for the majority of mucosal and systemic candidiasis. This study was conducted to determine the inhibitory effects of Dehydrozingerone, as a natural based material, on the biofilm formation in C. albicans.Methods:Dehydrozingerone was solved in DMSO and two-fold serial concentrations were prepared. C. albicans standard strain ATCC102331 was cultured on SDA medium, and spore suspension was prepared at a concentration of 3x107 CFU/ml. Fungal cells were planted in a flat-bottom 96-well microtiter plates with different concentrations of DHZ in RPMI medium as triplicate, and incubated for 48 h at 35С. After incubation time, planktonic cells were removed by discarding of upper medium and each well was washed with buffer (PBS) and fixed by methanol (99%), biofilm formation was measured using 0.4% crystal violet method. the absorbance was measured at 590 nm. The effect of DHZ on biofilm formation of C. albicans was calculated using determination of the percent transmission, the %r value of each test sample was subtracted from the %r of the reagent blank to obtain %r bloc.Results:The results indicated that the biofilm formation was inhibited dose-dependently with increasing the concentrations of dehydrozingerone. The biofilm formation was suppressed in the range of 22.5 to 50.34 % of DHZ with an IC50 equal 500 for tested concentrations after 48 h.Conclusion:Taken together, dehydrozingerone can be considered as a safe compound for potential use in preventing adherent and biofilm formation of C. albicans at oral candidiasis.

کلیدواژه ها:

Dehydrozingerone ، Candida albicans ، biofilm formation ، inhibition