PREVALENCE OF CARBAPENEMASE AND BLAKPC GENE IN KLEBSIELLA PNEUMONIAE STRAINS ISOLATED FROM ISFAHAN HOSPITALS, IRAN

Background and Aim:Klebsiella pneumoniae is a Gram-negative opportunistic pathogen. The carbapenems are effective therapeutic choice for the treatment of Klebsiella pneumonia infections. Carbapenemases are a group of enzymes capable of hydrolyzing carbapenems. This study was to introduce phenotypic and genotypic methods to identify the carbapenemase-producing isolates of Klebsiella pneumoniae.Methods:this study was to introduce phenotypic and genotypic methods to identify the carbapenemase-producing isolates of Klebsiella pneumoniae. The Modified Hodge Test (MHT) was performed to determine the susceptibility of isolates to antibiotics. The final products of PCR were electrophoresed on agarose gel.Results:The highest rate of resistance were observed for piperacillin (84%) and the lowest for ertapenem (50%). The majority of MHT positive isolates was from urine (64.7%), while abdominal and cerebrospinal fluids (0%) were the lowest. In addition, the ICU wards with 47 (69.1%) and the emergency units with 4 (5.9%) samples, had the most and the least frequent cases, respectively. MHT was positive in 68 K. pneumoniae isolates, but none of them were positive for blaKPC geneConclusion:The blaKPC gene has low prevalence in the Isfahan City, Iran.

کلیدواژه ها:

Klebsiella pneumonia ، Carbapenemase ، PCR ، Prevalence