EVALUATION OF THE PRODUCTION RATE OF EXTENDED-SPECTRUM BETA-LACTAMASE ENZYMES BY GRAM-NEGATIVE BACTERIA IN DIFFERENT CLINICAL SAMPLES ISOLATED FROM PATIENTS

Background and Aim:Treatment of infections depends on the sensitivity of these bacteria to antibiotic drugs. Therefore, the aim of this study was to detect the production rate of ESBL enzymes by bacteria from clinical samples isolated from patients.Methods:This descriptive- cross-sectional study was conducted during a one-month period in March, 2017. The study population included all clinical samples isolated from Toohid Medical Center, Sanandaj, Kurdistan province, Iran.. Combined disk method using 30µg of ceftoxime and cefotaxime30µg/clavulanic acid 10 µg was used to detect strains producing ESBL enzymes in different bacteria. According to the CLSI, if the growth halo around the cefotaxime/ clavulanic acid was ≥5 mm apart than cefotaxime alone, the strain is considered to be an enzyme producer. Data were analyzed by SPSS16 and frequency and ANOVA method (p<0.05).Results:Thirty seven clinical specimens, including bacteria producing ESBL enzymes, were observed. Of these, 31 (83.78%) were urinary culture. The bacteria producing the enzyme isolated from urine culture included 5 Klebsiella genus (16.12%) and 26 (83.87%) of Escherichia coli strains. Of 4 cases of lung secretion, 3 (75%) Klebsiella genus and 1 (25%) Acinetobacter genus was isolate. Of the two collected ulcers specimens, all 2 (100%) samples, including Klebsiella genus and producing ESBL. Also 6/37 (16.21%) samples were isolated from patients with nosocomial infections. There was no significant relationship between the production of this enzyme and the type of sample (p> 0.05).Conclusion:The prevalence ESBL-producing bacteria in patients with were observed. Appropriate methods to prevent of this problem should be considered.

کلیدواژه ها:

Extended-spectrum beta-lactamase ، gram-negative bacteria ، clinical samples ، patients