MOLECULAR EPIDEMIOLOGY OF PVL HARBORING HOSPITAL-ASSOCIATED METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS IN SEPTICEMIC CHILDREN, NORTHEASTERN IRAN, BOJNURD

Background and Aim:Methicillin Resistant Staphylococcus aureus (MRSA) is responsible for an increasing number of serious hospital and community acquired infections in adults and children. Sepsis caused by Staphylococcus aureus is one of the major health problems associated with treatment failure in adults, but its clinical outcomes, rate of treatment failure and its molecular epidemiology is poorly understood.Methods:We focused on Staphylococcus aureus strains isolated from children blood cultures in Bojnurd, Northeastern Iran. Totally, 58 S. aureus strains were isolated from blood cultures in major teaching hospital in Bojnurd. After primary verification of Methicillin resistance by agar screening method, the isolated MRSA strains were confirmed with detection of mecA gene. mecA positive strains evaluated for SCCmec, agr, and toxin profile. PVL positive isolates were subjected to evaluation of spa and Sequence Type.Results:Our data indicate 53.4% (31) of isolates were MRSA. 38.7% (12) of these isolates had PVL gene that 25% (3) of them had tsst-1 gene and 58.3% (7) had etb gene. 3.2% (1), 64.5% (20) and 32.2% (10) of these isolates belonged to SCCmec I, III and IV respectively. Predominant Sequence Type (ST) and spa types among PVL positive isolates were ST6 and t304 respectively.Conclusion:We had an uncommon finding, because PVL was routinely found in Community- Associated MRSA, but in this study we found PVL harboring Hospital-Associated MRSAs. A notable point about these isolates is that most of them belonged to Asian Endemic clones.

کلیدواژه ها:

Staphylococcus aureus ، Methicillin ، Resistance ، PVL ، MLST ، spa type ، SCCmec ، agr ، Children