RECOMBINANT ISDE PROTEIN OF STAPHYLOCOCCUS AUREUS: A NOVEL VACCINE CANDIDATE VERSUS MRSA

Background and Aim:Staphylococcus aureus is one of the dangerous pathogens with resistance against antibiotics especially β-lactams. With raising methicillin resistance Staphylococcus aureus (MRSA) in the world, immunotherapy approach was developed to eradicate such infections. Herein, immunogenicity of IsdE antigen was evaluated as a vaccine candidate in Balb/C mouse model.Methods:Recombinant IsdE protein was produced and evaluated by SDS-page and western blot and then formulated in Freund s’ and MF59 adjuvants to immunize Balb/C mice. Cytokines IFN-γ, IL-2, IL-4, IL-17 and TNF-α level and total IgG, IgG1 and IgG2a antibodies were assessed by ELISA.Results:Result of IFN-γ cytokine showed a significant increase in IsdE/MF59 group versus mere IsdE and PBS groups. Results of IL-2 demonstrated a remarkable increase in IsdE/Freund s’ group versus PBS group. Also, IL-17 cytokine cause significantly increases in IsdE/Freund s’ versus IsdE/MF59 group. Result of IL-4 cytokine showed that there was a remarkable increase in IsdE/MF59 versus mere IsdE antigen group. Result of TNF-α cytokine showed significantly increase in IsdE/MF59 group versus PBS group. Results of total IgG showed remarkable increase in IsdE /MF59 group versus mere IsdE group at dilutions of 1/00 and 1/200. Also a significant increase in IgG1 and IgG2a levels in experimental vaccine group which received IsdE /MF59 group as compared to the mere IsdE antigen and PBS group.Conclusion:These results may indicate the capacity of IsdE in the induction of cellular and humoral immune responses and also as a candidate vaccine to control the MRSA infections.

کلیدواژه ها:

IsdE protein ، Vaccine ، MRSA