PREVALENCE OF NONTUBERCLOSIS MYCOBACTERIA IN PATIENTS WITH SUSPECTED PULMONARY TUBERCULOSIS IN TEHRAN,IRAN

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 530

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شناسه ملی سند علمی:

MEDISM19_004

تاریخ نمایه سازی: 13 مهر 1397

چکیده مقاله:

Background and Aim:Nontuberculous mycobacteria(NTM) can be a cause of a variety of diseases that can not be distinguished from tuberculosis (TB) and is a diagnostic and therapeutic challenge. The purpose of this study was to isolate Nontuberculous mycobacteria from patients suspected to having pulmonary tuberculosis referring to Tehran regional tuberculosis laboratory.Methods:To identify NTM isolated from patients suspected of pulmonary tuberculosis at the species level, phenotypic tests including the method of Petroff method (NaOH 2%) as well as biochemical test were applied on the Loon Stein Johnson media. Also from colonies the DNA was extracted and PCR was performed for interested genes.Results:Overall 62 NTM strains were isolated from studied clinical samples were taken from TB suspected patients. Mycobacterium simiae(22.5%) was the most common isolated NTM followed by Mycobacterium fortuitum (16.1%), Mycobacterium kansasii (12.9%) and M. avium complex (6.4%).Conclusion:Our data showed that a significant number of tuberculosis cases were caused by NTM strains, among which Mycobacterium simiae has the highest prevalence. M. simiae can cause the same illness as tuberculosis, which is a serious problem for public health and attracts the attention of health officials, physicians and microbiologists.

کلیدواژه ها:

Nontuberculous mycobacteria / phenotypic method / molecular method / prevalence rate

نویسندگان

Zahra Nipour

Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Islamshahr Branch, Islamshahr, Iran

Mohammad Javad Nasiri

Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences

Hossein Dabiri

Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences

Maryam Ghane

Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Islamshahr Branch, Islamshahr, Iran