High fat diets alter aerobic exercise and L-arginine effects in ischemia reperfusion induced renal injury in rats: gender related difference

Introduction: Renal ischemia reperfusion (I/R) caused kidney injury gender dependently.High fat diet (HFD) contributes the development of renal dysfunction. L-arginine (L-arg)and regular exercise are recognized to be protective in I/R and lipotoxicity. We comparedthe role of aerobic exercise and L-arg supplementation against renal I/R in male and femalerats fed with HFD.Methods: 54 adult male and female Wistar rats received standard control diet (control),HFD, HFD plus L-arg (HFD±L-arg) or HFD plus aerobic exercise (HFD±EX) for 8weeks. Then the animals were subjected to renal I/R by clamping renal vessels for periodof 45 min followed by 24 hour reperfusion.Results: The serum levels of blood urea nitrogen (BUN) and creatinine (Cr), and kidneytissue damage score (KTDS) were not significantly different between HFD and controlgroups in two genders. However, the serum level of nitrite and kidney tissue level ofmalondialdehyde (MDA) in HFD fed male rats increased significantly (P<0.05). Also,kidney weight (KW) had significant decrement in HFD groups in comparison with controlgroups in two genders (P<0.05). L-arg and aerobic exercise decreased the BUN levels andKTDS in male rats after renal I/R (P<0.05), but such observations were not seen in female.Conclusion: These results indicated that L-arg and aerobic exercise could amelioraterenal I/R induced kidney injury in HFD male rats but not in female.

کلیدواژه ها:

Renal Ischemia Reperfusion ، High Fat Diet ، L-arginin ، Aerobic Exercise