Assessment of miRNA Expression data from human postmortem putamen samples in Parkinson’s disease

Background: For a long time, The dopamine (DA) precursor l-DOPA has been recognized as most effectivetreatment for Parkinson’s disease (PD). nevertheless, the response to this treatment changes by progression ofdisease, and motor deficits initiated in a few years of l-DOPA therapy in Parkinson s disease. However, themechanisms involved in molecular adaptations in the striatum are not well understood. miRNAs have importantroles in maintenance and development of striatal neurons by post-transcriptional regulating gene expression. Inthis article, the possibility that dysregulation of miRNAs response may participate in the etiology of Parkinson’sdisease is assessed.Methods: To examine miRNA function in human PD striatum, we used the integrative transcriptome analyses inGSE77667 by GEO2R, R, TargetScan and miRDB softwares. The functional pathways of these differentiallyexpressed miRNAs targeting genes were further analyzed with DAVID.Results: By analysis of GEOR, We found that 4miRNAs(miR-122, miR-155, miR-183 and miR-204) weresignificantly (p≤0.05) dysregulated in PD putamen when compared with control. We predicted the potentialtargets of the candidate miRNAs and realized that they are involved in 11 pathways. Our results discovered thatthe predicted potential targets of these microRNAs were significantly associated with PI3K-Akt and AMPKsignaling pathways.Conclusions: Altogether, our results proposed that in PD striatum, the differentially expressed miRNAs arerelated with the protein kinase signalling pathway. This mechanism may participate in the molecular adaptationsand associated motor complications found in PD.

کلیدواژه ها:

MicroRNA ، l-DOPA ، Parkinson’s disease