Hydroalcoholic extract of Anchusa italica injection protects against global cerebral ischemia– reperfusion injury via nitric oxide mechanism in rats
سال انتشار: 1396
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 404
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شناسه ملی سند علمی:
NGCMED10_123
تاریخ نمایه سازی: 16 تیر 1397
چکیده مقاله:
Introduction: In models of stroke, expression of the inducible nitric oxide synthase (iNOS) is responsible forcytotoxicity through the production of large amounts of nitric oxide (NO). Anchusa- italica is an importantmedicinal herb with anti-inflammatory, antioxidant and nerve protection. This study evaluates antioxidant effectof hydroalcoholic extract of Anchusa italics (HEAI) in the experimental stroke model in rat.Methods: at stroke model was induced by bilateral occlusion of both common carotid arteries for 60 min.HEAI (50, 100 mg/kg i.p.) was injected for 10 consecutive days. mRNA expression levels of the NOS subtypesand brain-derived neurotrophic factor (BDNF) in the hippocampus were analyzed by real-time PCR, as well asserum NO levels and serum and brain malondialdehyde (MDA) levels.Results: HEAI (50 and 100 mg/kg) groups decreased MDA in serum and the brain injury and increased theserum nitric oxide. In addition, HEAI (50 and 100 mg/kg) groups down-regulated mRNA expression of iNOSand up-regulated mRNA expression of eNOS and BDNF.Conclusion: The results indicate that administration of HEAI even after ischemia onset protects the brain fromfree radical injury, and inflammation via inhibiting nitric oxide and up-regulation of BDNF gene.
کلیدواژه ها:
Anchusa italica- stroke–reperfusion- rat-NO
نویسندگان
Samira Asgharzadeh
Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, hahrekord,Iran
Zahra Rabiei
Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
Mahmoud Rafieian-kopaei
Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran