Investigation of Malondialdehyde serum concentration and Antioxidant enzymes activity of patients with recurrent spontaneous abortion in Iranian women

Introduction: Malondialdehyde (MDA) is considered as an end product of lipid peroxidation and as an oxidative stress (OS) marker that is involved in various diseases such as recurrent spontaneous abortion (RSA). Paraoxonase and arylesterase are also known as anti-oxidant enzymes with different protective effects. The goal of the present study was to evaluate the association of the serum levels of MDA and activity of enzymes Paraoxonase and arylesterase with risk of RSA. Material and method: In this case- control study, 53 no pregnant women with RSA and 47 age, body mass index (BMI) and non- pregnant matched women as control group were enrolled. Level of MDA were determined in serum sample by using a calorimetrically method with thiobarbituric acid (TBA). The activity of enzymes paraoxonase and arylesterase were measured by spectrophotometry. Results: The mean serum level of MDA was significantly increased in RSA patients compared to a healthy control group (5.85 ± 1.00 vs. 5.47 ±0.77, P <0.05). We did not observe any significant difference between two groups in mean activity of enzymes Paraoxonase and arylesterase (P> 0.05, P> 0.05). We found a positive statisticalcorrelation between the serum levels of MDA and Anti paternal cytotoxic antibody (APCA) (r=+ 0.20, P= 0.04). Conclusion: Our study is the first study that showed, OS may have an elevation and subsequently play role in non- pregnant women with RSA. So, the enhancement of the OS in RSA women is not solely during pregnancy. However, more comprehensive studies are required to confirm our data

کلیدواژه ها:

Recurrent abortion ، Oxidative stress Malondialdehyde ، Paraoxonase ، Arylesterase