Insight To Ameliorative Effects of Insulin Against Apoptosis in Experimentally-Induced Diabetes Mellitus

Background: Diabetes Mellitus (DM) is a common metabolic and endocrine disorder that is characterized by hyperglycemia and insulin producing disability of beta cells. In this line the insulin-therapy is known as routine recommended medication. Accordingly, it has been illustrated that, the DM enhances reactive oxygen species (ROS)-related apoptosis in various tissues. Thus, the current study was designed to analyze the insulin-induced ameliorative effects against experimentally induced DM-related apoptosis at germ cells level.Method and Materials: To follow-up present study, 24 mature Wistar rats were divided into 3; Control, DM-induced (induced by single injection of 50mg/kg sterptozotocin, intraperitonealy) and DM-insulin treated (DMI) groups. After 56 days, the mRNA and protein levels of Bcl-2, Bax and Caspase-3 were analyzed by using reverse-transcriptase PCR (RT-PCR) and immunohistochemistry (IHC), respectively. To assess the positive reactions intensity, the pixel based frequency analyses and positive cells distribution per mm2 of testicular tissue sections were investigated. Results: Our results revealed that, the mRNA levels of Bcl-2 were significantly P<0.05) decreased in both DM and DMI groups versus control animals. Whereas, the protein levels of Bcl-2 were remarkably (P<0.05) elevated in both DM and DMI groups compared to control group. Moreover, the Bax and Caspase-3 expressions, in both mRNA and protein level, were significantly increased in DM and DMI groups.Conclusion: To wrap it up, although the insulin therapy remarkably ameliorates the pathologic effects of diabetes, the apoptosis ratio in DMI group still remains higher versus control animals

کلیدواژه ها:

Diabetes Mellitus ، Insulin ، Germ Cell ، Apoptosis