Effect of Inhibition of Mu Opioid Receptor in The VMH on Ovarian Cyst Formation By Morphine In The Rat

Backgournd: The main action of the opioid system is the relief of pain.Opium contains at least 20 types of alkaloids among which morphine is the most important alkaloids which make up about % 10-12. Studies have shown that morphine causes ovarian cysts.Morphine disrupts the ovarian cycle and may reduce fertility. The mu receptor plays a special role in various aspects of female reproduction. Removal of mu opioid receptors causes mice to be insensitive to morphine. In this study the aim is to use MgSO4 ornaloxone to inhibit the mu receptor engagement at the rat’s VMH. Methods: Female rats (200-250 g) kept under standard conditions. Using a Stereotactic device,they were surgically coordinated. Anteriorposterior: -1/92, venteral: 9, lateral: 0.5. After a week of recovery they were microinjected morphine (0/1-0/4 μg/rat, once intra VMH) and preinjected MgSO4 (1-5 μg/rat) and naloxone hydrochloride (0/1-0/4 μg/rat, once intra VMH) to inhibit mu receptor involvement. The control group received physiological saline (1 μL/rat, intra VMH). Three days after the experiment, uterus, ovary and brain samples were collected and studied histopathologically using hematoxylin.Results: The ovaries in group in which morphine was injected showed poly cystic features as compared to the control group. With the presence of MgSO4 or naloxone poly cystic ovary was not observed. Discussion & Conclusions: These results indicate that morphine disrupts fertility. This effect is most probably is resolved by mu opioid signaling blocking.

کلیدواژه ها:

Morphine ، Poly Cystic Ovary ، Mu Opioid Receptor ، Mgso4 ، Naloxone ، VMH ، Rat