Comparison of Mycoplasma Genitalium Diagnostic Primers in Vaginal Samples of Women With Recurrent Abortions

Background: There are several infectious agents that cause abortion and infertility. Mycoplasma genitalium is one of the most important pathogenic mycoplasmas in women and its detected quickly can be a solution to treatment and prevention. It has been different molecular methods and various primers for multiple target gene, developed to identify this bacterium. Purpose: The aim of this study was to evaluate the important primers for the detection and diagnosis of Mycoplasma genitalium by molecular methods in women with recurrent abortions. Research method: In this study, 100 samples of vaginal discharge from women were collected from their cervical area. DNA extraction from patient samples was performed using Boiling/DNG-PLUS. Two PCR tests were optimized on the standard strain. The tests were also examined for the limit of detection (LOD) and specificity. Results: In this study, two PCR tests optimized & amplicons 427bp and 335bp were amplified using specific primers of Mycoplasma genitalium. In specificity test, primers created bands only with DNA of Mycoplasma genitalium and there were no bands of DNA with other microorganisms. LOD for both tests were calculated 10 copy/reaction. Of 100 samples examined, for amplicon 427bp only one (1%), and for amplicon 335bp, seven samples were positive (7%). Discussion and Conclusion: between two important primers examined primer with a product of 335bp, was efficient more positive results were obtained which indicates that the primer is better than other primer. It is therefore justified in molecular diagnostic tests Mycoplasma genitalium more effective primer was used.

کلیدواژه ها:

Mycoplasma genitalium ، abortions ، Detection ، PCR