Study The Effect of N-Acetyl Cysteine (NAC) on Apoptotic Factors in Granulosa Cells of Women with Polycystic Ovarian Syndrome

Background: Polycystic Ovary Syndrome (PCOS) is a common endocrine disorder and one of the causes of infertility among women. Oxygen reactive species (ROS) can increases the number of atretic follicles, which occurs as a result of increasing of apoptosis in granulosa cells of the follicles. In this study, NAC was used as a potent antioxidant to reduce oxidative stress. Methods: 60women entered the study with specific Inclusion and exclusion criteria and divided into 3 groups of 20people. The first group consisted of 20women with PCOS who received NAC 600 mg, TDS for six weeks, The second group included 20 women with PCOS who received placebo TDS for six weeks and The third group included 20women with normal ovarian function and infertility problems due to male or mechanical factors. Follicular fluid was collected after oocyte puncturing and granulosa cells were isolated. By using the Real Time PCR method, the expression of the pro-apoptosis genes such as caspase-3, and the anti-apoptosis genes such as Bcl-2 were investigated in different groups. Results: The rate of expression of caspase-3 in granulosa cells of the NAC-treated group was lower than placebo and control group. Also, the expression of Bcl-2, in granulosa cells of the NAC-treated group was higher than placebo group and control group, Which indicated that apoptosis and apoptotic factors decreased in the group receiving NAC antioxidants. Conclusion: By reducing the level of oxidative stress, using a potent antioxidant such as NAC, apoptosis in granulosa cells in women with PCOS can be reduced

کلیدواژه ها:

Polycystic ovary syndrome ، Granulosa cells ، Apoptosis ، NAC