Effect of Curcumin on Fatty Acid Synthase expression and enzyme activity in breast cancer cell line SKBR3

Introduction: Fatty acid synthase is a multifunction protein that catalyzes de novo synthesis of longchain fatty acids. FASN expression is higher in HER2-positive cells, such as SKBR3 and MCF-7/HER2 cells, than in MCF-7 cells, which express lower HER2 levels. Curcumin, a yellow-colored hydrophobic polyphenol derived from the rhizome turmeric, significantly suppressed growth of human breast cancer cells. In this study, we assessed the effect of curcumin on expression and activity of fatty acid synthase in SK-BR-3 breast cancer cells.Aim: In this study, we decided to determine effect of Curcumin on Fatty Acid Synthase expression and enzyme activity in breast cancer cell line SKBR3.Materials and Methods: We assessed the cytotoxicity effect of curcumin in SK-BR-3 cells by MTT. Apoptosis was performed by flow cytometry. FAS activity was measured by a spectrophotometer at 340 nm of NADPH absorption. Fatty acid synthase gene expression was analyzed by real-time PCR Results: Curcumin could decrease cell viability and induce apoptosis in SK-BR-3 cells. Curcumin also reduces the enzyme activity and expression of fatty acid synthase. Conclusions: It is possible that inhibitory effect of curcumin on FAS may induce apoptosis in SK-BR-3 breast cancer cells

کلیدواژه ها:

fatty acid synthase ، curcumin ، breast cancer cells ، apoptosis