The effect of quercetin and resveratrol the expression of some genes involved epigenetic in breast cancer cell lines

Introduction & Aim: Breast cancer is one of the most common cancers among women. Incorrect pattern of gene expression involved in epigenetic Including APOBEC3B, DNMT-1 and TET_1 can develop breast cancer.Quercetin and resveratrol is a natural flavonoids that antioxidant and anti-cancer properties have been reported in other studies. To investigate the mechanism of effect of quercetin and resveratrol, this study examined effect of quercetinan resveratrol on the expression of genes referred to the two classes of breast cancer cell lines Methods: cell lines, including MCF-7 and MDA-MB-453 in separate boxes including the control group and treated groups with two doses of 50 and 100 micro molar of quercetin and two doses of 25 and 100 micro molar for 24 hours of resveratrol for 48 hours were cultured. RNA was extracted from the cells and then converted to cDNA. realtime PCR were used for APOBEC3B and DNMT and TET_1expression. Results: The results showed that quercetin and resveratrol decrease in gene expression APOBEC3B and DNMT-1 And increased expression of TET_1 in both of cell lines.Conclusion: Due to the satisfactory effects of quercetin and resveratrol has on breast cancer cells, probably these effects applied through epigenetic mechanisms. However, the final decision needs to be further investigation