Some genetic properties of Iraqi buffalo using polymerase chain reaction technique-PCR

The study aimed to study the genetic diversity of the Iraqi buffalo through the use of different techniques, first technique was PCR-RAPD technique and the second was microsatellite technique (PCR-STR). After extracting the DNA samples, seven primers of PCR-RAPD technique included C01, C02, C04, C06, C07, C09, C11 and C12. Primers of PCR-STR technique were ILSTS005, ILSTS029 and ILSTS072. The study highlights the usefulness of heterologous PCR-RAPD and microsatellite markers to assess the genetic variability in buffalo. Also various diversity indices suggest sufficient genetic variability within Iraqi buffalo that can be utilized as initial guidelines for future breeding strategies and conservation