Exportin5 gene polymorphism and recurrent pregnancy loss

Background: Unexplained recurrent pregnancy loss refers to three or more abortions before 20 weeks of gestation. Exportin5, is one of the crucial molecules that has been identified in the trophoblast cells of the placenta, which was involved in the biogenesis and processing of Micro-RNA. Human embryo produced a large number of micro RNAs, which were involved in fetal development. One of the important molecules that has been identified in the trophoblast cells of the placenta is Exportin5, involving the biogenesis and processing of micro RNA. Single nucleotide variation in Exportin5 gene (rs2257082;C> T) encoded could change the quality of micro-RNA processing and affect pregnancy outcome.Methods: In the present study, we included 100 women with unknown recurrent pregnancy loss as case group and 100 women that have an at least one live child without abortion history were selected as a control group. After DNA extraction, we applied PCR-RFLP method for molecular genotyping of this polymorphism between both groups.Result: Our findings revealed that the frequency of genotypes in the wild-type homozygote (CC), heterozygote (CT) and mutant homozygote (TT) of Exportin5 in patients and healthy women were 27%, 71%, 1% and 16%, 83%, 1%, respectively. Statistical analysis has shown significant differences in genotype frequencies between cases and control groups (p=0.045).Conclusion: The present study revealed that polymorphism gene of Exportin5 can be a predisposing factor for recurrent pregnancy loss, and also can be considered as a molecular biomarker in forewarning of recurrent pregnancy loss.

کلیدواژه ها:

Exportin5 ، Recurrent pregnancy loss ، Polymorphism