Effects of Conjugated Linoleic Acid (CLA) on spermatogenesis and apoptosis of germinal cells in male mice model of obesity

Background: Obesity, which has been increased in recent years, associates with reduction of the rate of fertility. Since CLA can reduce the obesity, the purpose of this study is to investigate the effects of CLA on spermatogenesis and apoptosis of germinal cells in male rat model of obesity induced by different oils.Methods: In this cross-sectional study, 60 male NMRI mice were used. They were randomly divided into 6 groups delivering different sources of oil: Group A (Fish oil), Group B (Olive oil), Group C (Hydrogenated sunflower oil), Group D (Flax oil), Group E (Dehydrogenated sunflower oil) and group F (Control). After 14 weeks of being on a diet of different oils, 5 mice of each group continued their diet of different oils and 5 remaining mice of each group were treated with CLA for 4 more weeks. Following 18 weeks, histological studies of testis for germinal epithelium quality and apoptosis in germinal cells were evaluated by H&E staining and TUNEL assay respectively. Data were analyzed by ANOVA test in SPSS software (16th version).Result: Olive oil significantly attenuated germinal epithelium (P=0.01), but other oils did not alter the germinal epithelium significantly. CLA treatment ameliorated adverse effects of olive oil (P=0.001), hydrogenated sunflower oil (P=0.05) and flax oil (P=0.03). Number of apoptotic cells was highest in the dehydrogenated sunflower oil compared to the control group (P=0.02). CLA treatment could significantly decrease the number of apoptotic cells in the dehydrogenated sunflower oil (P=0.01) and olive oil (P=0.03) groups.Conclusion: CLA treatment ameliorates the adverse effects of different oils on spermatogenesis and apoptosis of germinal cells

کلیدواژه ها:

apoptosis ، conjugated linoleic acid ، spermatogenesis ، obesity