L-NAME inhibitory effect on ovarian cysts induced by morphine after the administration of drug in mid-ventral hypothalamus of Wistar rats

Background: Polycystic ovary syndrome is a kind of complication in the reproductive system causing infertility. L- NAME is a competitive inhibitor for nitric oxide (NO) synthase enzyme which can antagonize the effects of NO, the pro-inflammatory mediator involved in cyst induction by morphine. This study examines the interaction of morphine / L-NAME in ventro-medial hypothalamus of Wistar rats.Methods: Animals were Wistar rats weighing 200 – 250 g used as virgin material. Morphine (0.001 to 0.4 micrograms / rat) was microinjected into the ventro-medial hypothalamus of rats (nucleus coordinates AP: -1.96). L NAME (0.1 to 0.4 micrograms / rat) was injected alone or pre-injected. In the latter process, L NAME (0.1 - 0.4 micrograms / rat) was administered prior to morphine (0.4 micrograms / rat). The precursor of nitric oxide (L-arginine- 0.1 to 0.4 micrograms / rat) in an independent process was injected into the nucleus alone or in competition with L-NAME. The control group received only saline. After completion of the experiments, the animals were anesthetized and after surgery, the ovaries were collected and investigated. Result: The ovaries of morphine creviecr rats showed polycystic view .The number of cysts was significantly decreased in samples pretreated L-NAME. Also reducing effect on cyst by L-NAME intervention in the group receiving L-arginine was obtained.Conclusion: Probably the polycystic ovary syndrome is induced in rat due to interaction of opioid receptors and morphine at the core of the mid-ventral hypothalamus and because of activation of enzyme NO synthase; the problem which is stopped by inhibition of this enzyme.

کلیدواژه ها:

EMAN –L ، Polycystic ovary ، Rats ، Morphine