The effect of hydro-alcoholic extract of lamium album on the ratio of Bax/Bcl-2 and telomerase activity in HeLa cells

Background: Lamium album, commonly called white nettle is widely used in medical setting especially in traditional healing ways. Previous studies have been reported considerable cytotoxicity, pro-apoptotic and antitumor effects of Lamium album. HeLa is one of the cervical cancer cell lines in which hydro-alcoholic extract of Lamium album effects have not been studied so far. The aim of this study was to investigate the effects of hydro-alcoholic extract of Lamium album on HeLa cervical cancer cell line.Methods: HeLa cells were cultured in RPMI 1640 medium at 370C in humidified condition of 95% and 5% CO2 incubator. Then, cells transferred to 9 plates with various concentration of hydro-alcoholic extract (0-32 mM) and treated for 72 hours. Telomerase activity was determined by TRAP assay method, also Bax and Bcl-2 protiens concentrations were determined by ELISA method.Result: The cytotoxic effects of different concentrations of Lamium album on HeLa were observed as a reduction of telomerase activity and altered the expression of Bax/Bcl-2 ratio. Also, results showed that there are significant difference between telomerase activity decreasing and increase in Bax/Bcl-2 (P=0.001).Conclusion: The current study demonstrated that hydro-alcoholic extract of Lamium album can reduce HeLa cell viability by reducing telomerase activity and increasing the rate of Bax/Bcl-2. It is concluded from this study that Lamium album can be used as an anti-cancer drug for cervical cancer cell lines through inhibition of telomerase activity and induction of apoptosis.

کلیدواژه ها:

Bax ، Bcl-2 ، HeLa ، Telomerase activity ، Lamium album