Human Wharton’s jelly mesenchymal stem cells can be an ideal cell source for cell therapy protocols in reproductive biomedicine

Background: WJ-MSCs derived from proximal epiblast in day 13 of the embryogenesis have same origin with PGCs, so it supposed WJ-MSCs may have some characteristics of germ cells. The purpose of present study was to evaluate germ cell marker expression in WJ-MSCs.Methods: WJ-MSCs were isolated through explant method then characterized by flowcytometry in the third passage also differentiated to adipocytes and osteocytes. Then WJ-MSCs were cultured in α-MEM containing 10% FBS for 21 days. Gene expression of ZP1, ZP2, ZP3, GDF-9, VASA, C-KIT, and SYCP3 (oocyte and germ cells specific markers) were evaluated under Real-Time PCR analysis on days 0, 7, 14 and 21 of culturing. Also on day 21 ZP3, VASA, and SYCP3 proteins were investigated by immunofleurescent assay.Result: Flowcytometry analysis indicated that isolated cells could express CD73, CD90 and CD105 (MSCs markers) but don’t express CD34 and CD45 (hematopoietic markers) and also could differentiate into adipocytes and osteocytes. Immunofleurescent technique and Real time PCR analysis revealed WJ-MSCs could express oocyte and germ cells specific markers.Conclusion: The present study demonstrated that WJ-MSCs could express oocyte and germ cells markers at low levels without no differentiation medium, so it showed that WJ-MSCs could maintain their germ cell memory. In accordance to this capability of WJ-MSCs, it seems they could provide prominent source for cell therapy protocols in reproductive biomedicine specially for those who have deficiency or lack of germ cells.

کلیدواژه ها:

Herm cells memory ، Proximal epiblast ، Reproductive biomedicine ، WJ-MSCs