The protective effect of royal jelly on embryo devolopment against paternal heat stress induced embryo hazard

Background: Scrotal hyperthermia has been known as a cause of male fertility and recent study suggested that scrotal hyperthermia by exposure to water bath can induce a significant alteration sperm fertilization capacity and embryo development. The present study aimed to evaluate the protective effect of Royal jelly (RJ) on embryo development rate alterations following heat stress in male rats.Methods: In this study, 40 adult male rat were randomly divided into the 8 groups (n=5): control, RJ, 43℃ ,39℃ ,37℃ , 43℃+RJ ,39℃+RJ , 37℃+RJ (these rats heated in water bath for 20 mins) .RJ, at a dose of 100 mg/kg BW was given by gavage. The duration of treatment was 48 days. At the end of treatment period the rats were sacrificed by dislocation of cervical vertebrae. After removal of testis the tail of epididymis was isolated and transferred to HTF medium. Percentage of zygotes, two cell, morula, blastocyst and type of embryos were evaluated. Data were analyzed using one way ANOVA and tukey test.Result: Our data indicate decreased significantly (p Conclusion: These results demonstrate that paternal heat stress significantly reduces the development of preimplantion embryos.

کلیدواژه ها:

Embryo ، In vitro fertilization ، Royal jelly ، Heat stress