The Protective effect of royal jelly on the development in vitro of preimplantation embryos against nicotine- induced embryo toxicity in male mice

Background: Nicotine is responsible for many harmful effects of smoking on reproductive health in several ways including alteration in the fertility of both men and women, in gestation, and in embryo development. The aim of present study is to assess protective or ameliorative effect of Royal jelly (RJ) on in vitro fertilization among nicotine induced embryo toxicity in male mice.Methods: 36 male balb/c mice weighing 25-30 g were randomly divided into six groups(n=6). Group 1 received 0.2 normal salin (control), group 2 received 100 mg/kg RJ, group 3 and 4 received 0.5 mg/kg and 1.0 mg/kg of nicotine, respectively, group 5 received 0.5 mg/kg of nicotine with 100 mg/kg of RJ and group 6 received 1.0 mg/kg nicotine and 100 mg/kg RJ. All administration were done orally. After 30 days, percentage of zygotes, two cell, blastocyst and type of embryos were evaluated and the data were analyzed using one way ANOVA.Result: Results indicated significantly reduce in the percentage of fertilized oocyte, two cell embryo, morula, blastocyst and arrested embryos in nicotine treated mice in comparison with control and royal jelly groups. However, in groups that received nicotine with royal jelly all of these parameters were ameliorated significantly (pConclusion: Our data suggests that nicotine- induced development toxicities could be protective or ameliorated by royal jelly.

کلیدواژه ها:

embryo ، IVF ، Royal jelly ، Nicotine