Inhibiting cell proliferation by silymarin fairly inhibits endometriotic grafts development: Evidences for GDNF, GFRα1 and Bcl6 genes expression

Background: Endometriosis (EMS), a common obstetrics disorder, is determined by the presence of endometrial structures outside the uterine cavity. The inflicting roles of inflammation and oxidative stress in promoting the cellularity in endometriotic tissues have been established well. Minding the anti-inflammatory and antioxidant effect of silymarin (SMN) and considering the core roles of GDNF, GFRα1 and Bcl6 in cell proliferation, the present study aimed to uncover the inhibitory effect of SMN on these genes expression.Methods: Experimental endometriosis was induced in 14 female Wistar rats and then the animals were randomly divided into 2 groups, including endometriosis-sole group (No:7) and endometriosis-induced SMN-treated group (No:7). The SMN was administrated at dose level of 0.7mg/kg, every two days, orally (by gavages). Following 30 days, the animals were euthanized and ectopic auto-grafted endometriums were sampled. The mRNA levels of GDNF, GFRα1 and Bcl6 were assessed by using RT-PCR and the immunohistochemistry (IHC) was used to estimate positive cells distribution/one mm2 of the tissue.Result: The animals in SMN-treated group exhibited a significant (PConclusion: Our data suggest that, the SMN potentially inhibits grafts development by suppressing cell proliferation machinery, including GDNF, GFRα1 and Bcl6.

کلیدواژه ها:

BCL6 ، Endometriosis ، GDNF ، GFRα1 ، Rat ، Silymarin