Evaluation of oxidative stress in the polycystic ovarian rats

Background: The findings about evaluation of oxidative stress in the poly cystic ovarian syndrome (PCOS) patients are conflicted. The aim of this study was evaluation of malondialdehyde (MDA) and total antioxidant capacity (TAC) levels as two biomarkers of oxidative stress in a rat model of polycystic ovarian syndrome.Methods: Ten female rats (21 days of age) were divided into 2groups as follows: 1) The Sham group that had no treatment. 2) The PCO-Model group. In this group testosterone enanthate 1mg/100 g body weight was injected for 35 days once daily to induction of polycystic ovary phenotype. After treatments and obtaining serum samples the fasting levels of MDA, asured by using a commercially kit available to quantitative assay antioxidant capacity on the basis of the oxidation reduction colorimetric assay. The MDA levels were determined by using an Agilent Technologies 1200 Series HPLC system. All analysis was directed by Student’s t-test using Graph Pad Prism software version 5, 04 and data were shown as the mean ± SEM.Result: The mean of serum MDA levels in PCO-Model rats were higher than the Sham rats (p Conclusion: Our findings suggest that induction of PCOS by testosterone enanthate in rats can affect oxidation state and causes an increase in MDA level as a biomarker of lipid peroxidation.

کلیدواژه ها:

Malondialdehyde ، Polycystic ovary ، Rat ، Testosterone ، Total antioxidant capacity ، Oxidative stress