Evaluation of VEGF gene polymorphism in Iranian women with endometriosis

Background: Endometriosis is a most common benign gynecological disease which affects women of reproductive age. It is characterized by the presence of the endometrium outside the uterine cavity, leading to infertility, pelvic pain, and dysmenorrhea. Genetic, endocrine, immunological, and environmental factors have been suggested in its pathogenesis. It is a multi-factorial and polygenic disease in which angiogenesis may be implicated. Angiogenesis is under the control of numerous inducers, including vascular endothelial growth factor (VEGF).Vascular endothelial growth factor (VEGF) is an endothelial cell-specific angiogenic protein suspected to be involved in the pathogenesis of endometriosis. The aim of the present study was to assess the role of the vascular endothelial growth factor (VEGF) -2549 insertion/deletion (I/D) polymorphism in susceptibility to endometriosis.Methods: This study comprised 100 patients who had a histologically confirmed diagnosis of endometriosis (cases), and 200 healthy women without evidence of the disease (controls). VEGF -2549 I/D polymorphism was determined using polymerase chain reaction (PCR). Genotyping for the -2549 I/D polymorphism was performed using the forward 5 -GCTGAGGATGGGGCTGACTAGGTA-3 and reverse 5 - GTTTCTGACCTGGCTATTTCCAGG-3 primers.Result: The frequency of the II, ID, and DD genotype was 14 versus 17.5%, 52 versus 50%, and 34 versus 32.5%, in patients and controls, respectively. A statistically significant difference was not observed for genotype distribution among the patients and controls (p = 0.3).Conclusion: Our results suggest that -2549 I/D polymorphismof VEGF gene did not appear to have an influence on endometriosis susceptibility in a Iranian population.

کلیدواژه ها:

Endometriosis ، Gene ، Polymorphism ، Susceptibility ، VEGF