Investigation the effect of Achillea millefolium inflorescences extract on DNA damage of sperms in cyclophosphamide treated mice

Background: Apart from being an immunosuppressant agent, cyclophosphamide (CYP) has been known to induce oxidative stress, impact on gonadal cells’ DNA and reduce the fertilizing potential. Therefore, the present study was aimed to evaluate the effects of hydro-alcoholic extract of Achillea millefolium inflorescences (AMI), as a potential antioxidant on DNA damage in CYP treated mice.Methods: Thirty male adult NMRI mice were randomly arranged into 5 groups. Group 1 received normal saline (0.1 ml/kg), group 2 received CYP alone (5mg/kg), group 3 received CYP (5mg/kg) + hydro-alcoholic extract of AMI (75mg/kg). Group 4 received CYP (5mg/kg) + hydro-alcoholic extract of AMI (150mg/kg) and Group 5 received CYP (5mg/kg) + hydro-alcoholic extract of AMI (300mg/kg). Treatments were continued for 35 days. At the end, after mice euthanization by cervical dislocation, Caudaepididymis were used to collect sperm cells and rate of DNA damage were examined by Acridine Orange Staining. Statistical analyses were performed using ANOVA and Tukey test.Result: In group 2, the DNA damage significantly increased compared to control group (pConclusion: These findings indicated that AMI (low dose) has protective effect against CYP-induced toxicity in CYP treated mice probably by decreasing oxidative stresses. But, high dose of AMI caused increase toxicity of CYP.

کلیدواژه ها:

Acridine orange ، Cyclophosphamide ، DNA damage ، Mice ، Achillea millefolium