The effect of copper oxide nanoparticles on the expression of TSPY genes affecting rats testicular tissue

Background: Considering the increasing use of nanoparticles in recent years, there are wide spread concerns about the potential risks of nanoparticles containing the chemical release into the environment, including environmental toxicity, cytotoxicity, infertility and cancer. TSPY gene is expressed in testicular, and its proteins is involved in spermatogenesis. This gene seems to determine the spermatogenesis time, by sending signals to spermatogonia to enter meiosis. This study was conducted to evaluate the effect of copper oxide nanoparticles on TSPY gene expression in testis and its impact on the fertility.Methods: In this experimental study, the number of 15 adult male rats of the Wistar, in three treatment groups, received intraperitoneal injection copper oxide (CuO) with a size of 60-20 nm, purity 99, concentration of 0/75 mg / ml, 0/5, 0/25 for 14 days. Similarly, the control group received normal saline injection. After deep anesthesia, the rats were autopsied. And to extract RNA, 100 mg was removed from testicular tissue, and RNA extraction (by Protocol kit Iran, Molecular Biology Products Denazist Asia) and cDNA synthesis (By Kit Fermentas ) was performed. Ultimately, the amount of change in gene expression was examined by PCR real time techniques.Result: The results of real time PCR showed a significant decrease in gene expression of treatment groups compared to the control group.Conclusion: The findings of this study showed that, the copper oxide nanoparticles can reduce the expression of genes which are associated with male fertility.

کلیدواژه ها:

Gene ، Nanoparticles ، Rat ، Testicle