The effect of equine Chorionic Gonadotropin on in vitro calf spermatogonial stem cell culture

Background: The complex process of spermatogenesis is regulated by various factors. Studies on spermatogonial stem cells (SCCs) have provided very important tool to improve herd genetic and different field. 0.2 to 0.3 percent of total cells of seminiferous tubules consist of spermatogonial stem cells. To investigate the biomanipulation of these cells, proliferation and viability rate of cells should be increased in vitro, at first. Equine chorionic gonadotropin (eCG) has been suggested to play a determinant role in the survival of germ cells in addition to increasing spermatogonial proliferation.Methods: In this study, the in vitro effects of eCG on spermatogonial cell colony formation were investigated. Sertoli and spermatogonial cells were isolated from 3-5 months old calves. The identity of the Sertoli cells and spermatogonial stem cells were confirmed through immunocytochemistry and colony morphology, respectively. Co-cultured Sertoli and spermatogonial cells were treated with eCG in different doses of 2, 5 and 10 IU mL-1 eCG, before colony assay.Result: Results indicated that, eCG increased in vitro colonization and prolifrationof spermatogonial cells in comparison with control group.Conclusion: In conclusion, using eCG provided proper bovine spermatogonial stem cell culture medium for in vitro study of these cells.

کلیدواژه ها:

eCG ، Sertoli cell ، Bovine spermatogonial stem cell