Genetic variant of HDAC3 and idiopathic male infertility in a population in Iran

Background: In humans, approximately 15% of couples experience some form of infertility and 50% of these infertile couples can be ascribed to the male factor. More than 3000 genes are considered to be involved in male s spermatogenesis. HDAC3 gene is located on 5q21 and encodes a protein with 428 amino acids. Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. The mechanism of action of the HDAC involves removing the acetyl group from the histones comprising the nucleosome. HDAC3 is expressed at high levels in Sertoli and germ cells. HDACs must somehow change qualitatively or quantitatively during spermyogenesis. HDAC3 is an essential component for the coordination of histone modification profiles to regulate specific gene expression profiles. In addition to histone targets, HDAC3 can deacetylate other proteins and regulate their localization and activity. The aim of this study was to examine the association of HDAC3 rs2547547gene variety with male infertility.Methods: Blood samples were collected from infertile men and healthy controls and were analyzed by using PCR-RFLP method.Result: Allele and genotype distribution did not differ significantly between patients and controls (P=0.72).Conclusion: In conclusion, the results of this study indicate that SNP HDAC3 rs2547547gene may not be associated with male infertility in this population. However, more studies should be considered with larger number of patients and control for clarifying the results.

کلیدواژه ها:

Male infertility ، Polymorphism ، Spermatogenesis ، HDAC3